Coding
hpd

Part:BBa_K1747013:Design

Designed by: Harry Bennett   Group: iGEM15_LaVerne-Leos   (2015-08-27)


hpd gene from Arabidopsis thaliana


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 895
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 490


Design Notes

The La Verne Leos team had to order a g-block for this part. An illegal EcoR1 site was removed by introducing a point mutation that still codes for the same amino acid.

Source

http://aem.asm.org/content/71/10/5678.full

References