Coding
hpd
Part:BBa_K1747013:Design
Designed by: Harry Bennett Group: iGEM15_LaVerne-Leos (2015-08-27)
hpd gene from Arabidopsis thaliana
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 895
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 490
Design Notes
The La Verne Leos team had to order a g-block for this part. An illegal EcoR1 site was removed by introducing a point mutation that still codes for the same amino acid.
Source
http://aem.asm.org/content/71/10/5678.full